DOT Drug Testing Done

Right. Every Time.

Federally compliant drug testing for CDL drivers, transportation companies, and all DOT- regulated employees conducted under 49 CFR Part 40 by a certified collector, at your location.

DOT Drug Testing Done

Right. Every Time.

Federally compliant drug testing for CDL drivers, transportation companies, and all DOT-

regulated employees conducted under 49 CFR Part 40 by a certified collector,

at your location.

(000) 000-0000 — Available 24/7

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Trusted By:

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What Is a DOT Drug Test?

The Department of Transportation (DOT) requires drug and alcohol testing for employees in safety-sensitive positions across several federally regulated industries.

These tests are mandated by federal law and must follow strict guidelines outlined in 49 CFR Part 40 the federal regulation that governs how DOT workplace drug and alcohol testing programs must be conducted.

Failure to comply with DOT drug testing requirements can result in significant fines, loss of operating authority, and serious liability exposure.

At Luxe Mobile Lab Screening, every DOT collection is conducted by a certified collector following exact federal protocols so your program stays compliant and your documentation holds up to any audit or review.

What Is a DOT Drug Test?

The Department of Transportation (DOT) requires drug and alcohol testing for employees in safety-sensitive positions across several federally regulated industries.

These tests are mandated by federal law and must follow strict guidelines outlined in 49 CFR Part 40 the federal regulation that governs how DOT workplace drug and alcohol testing programs must be conducted.

Failure to comply with DOT drug testing requirements can result in significant fines, loss of operating authority, and serious liability exposure.

At Luxe Mobile Lab Screening, every DOT collection is conducted by a certified collector following exact federal protocols so your program stays compliant and your documentation holds up to any audit or review.

The Test

What the DOT Drug Test Screens For

The DOT 5-panel urine drug test is standardized by federal regulation. Every DOT test screens for the same five substance categories, regardless of employer or industry.

Price:

$85

per test

The 5 Panels:

  • Marijuana (THC): Including metabolites

  • Cocaine: Including metabolites

  • Amphetamines: Amphetamine, methamphetamine, MDMA, MDA

  • Opiates: Codeine, morphine, heroin (6-MAM)

  • PCP: Phencyclidine

Collection Method:

Urine only as required by federal DOT regulations

Result Turnaround:

Typically 24–48 hours after laboratory receipt

MRO Review:

Reviewed by a Medical

Review Officer

Documentation:

Federal CCF completed for every test

The Test

What the DOT Drug Test

Screens For

The DOT 5-panel urine drug test is standardized by federal regulation. Every

DOT test screens for the same five substance categories, regardless of

employer or industry.

Price:

$85

per test

The 5 Panels:

  • Marijuana (THC): Including metabolites

  • Cocaine: Including metabolites

  • Amphetamines: Amphetamine, methamphetamine, MDMA, MDA

  • Opiates: Codeine, morphine, heroin (6-MAM)

  • PCP: Phencyclidine

Collection Method:

Urine only as required by federal DOT regulations

Result Turnaround:

Typically 24–48 hours after laboratory receipt

MRO Review:

Reviewed by a Medical

Review Officer

Documentation:

Federal CCF completed for every test

When Testing Is Required

Every Situation Covered

DOT regulations require testing in six specific situations. We handle all of them including emergency and after-hours collections.

Pre-Employment Testing

Required before a CDL driver or safety-sensitive employee begins performing DOT-regulated duties. No driver may operate a commercial motor vehicle until a pre-employment test is completed with a verified negative result.

Random Drug Testing

DOT-regulated employers must conduct random, unannounced testing at federally mandated rates. We manage consortium enrollment and collector dispatch so you stay compliant year-round without the administrative burden.

Post-Accident Testing

Following certain accidents involving a commercial vehicle, DOT requires testing within specific time windows 8 hours for alcohol, 32 hours for drugs. We are on

call 24/7 to ensure you meet these deadlines.

Reasonable Suspicion Testing

When a trained supervisor observes signs and symptoms of drug or alcohol use, testing must be conducted. We respond quickly and discreetly to protect your program's integrity.

Return-to-Duty Testing

Before a driver who violated DOT drug or alcohol rules can return to safety-sensitive duties, a test with a verified negative result is required. We coordinate directly with your Substance Abuse Professional.

Follow-Up Testing

After a return-to-duty test, DOT requires unannounced follow-up testing for a minimum of 12 months. We manage the schedule and dispatch so nothing falls through the cracks.

When Testing Is Required

Every Situation Covered

DOT regulations require testing in six specific situations. We handle all of them including

emergency and after-hours collections.

Pre-Employment Testing

Required before a CDL driver or safety-sensitive employee begins performing DOT-regulated duties. No driver may operate a commercial motor vehicle until a pre-employment test is completed with a verified negative result.

Random Drug Testing

DOT-regulated employers must conduct random, unannounced testing at federally mandated rates. We manage consortium enrollment and collector dispatch so you stay compliant year-round without the administrative burden.

Post-Accident Testing

Following certain accidents involving a commercial vehicle, DOT requires testing within specific time windows 8 hours for alcohol, 32 hours for drugs. We are on

call 24/7 to ensure you meet these deadlines.

Reasonable Suspicion Testing

When a trained supervisor observes signs and symptoms of drug or alcohol use, testing must be conducted. We respond quickly and discreetly to protect your program's integrity.

Return-to-Duty Testing

Before a driver who violated DOT drug or alcohol rules can return to safety-sensitive duties, a test with a verified negative result is required. We coordinate directly with your Substance Abuse Professional.

Follow-Up Testing

After a return-to-duty test, DOT requires unannounced follow-up testing for a minimum of 12 months. We manage the schedule and dispatch so nothing falls through the cracks.

Industries We Serve

Built for Every DOT-Regulated Industry

DOT Drug Testing

Transparent Pricing

DOT Testing Fees

Straightforward pricing with fully broken-down variables. We follow absolute regulatory

transparency with no hidden administration or reporting surcharges.

Testing Methods — Luxe Mobile Lab Screening
$85
DOT Drug Test

Federally regulated 5-panel urine drug test. Includes CCF documentation, certified collection, and MRO review on positive results.

+$75
After-Hours / Emergency

Available outside normal business hours for post-accident and urgent situations. 24/7 on-call availability.

+$50
Weekend Service
Fee

Saturday and Sunday appointments available with advance scheduling or on-call for emergencies.

$0.70
Mobile Mileage
Fee

Calculated from our Henry County location to your testing site and back. Applied to all mobile appointments.

How It Works

Stress-Free DOT

Compliance In 4 Steps

1
Step 1
Schedule Your Test
Call, text, or book online. Tell us the type of DOT test required, your location, and the time window. Same-day and after-hours available.
Step 2
Certified Collector Arrives
A DOT-certified collector arrives at your workplace, fleet yard, or location of choice — fully equipped with all required federal documentation.
2
3
Step 3
Federal Collection Protocols
Specimen collected following exact 49 CFR Part 40 procedures. Federal CCF form completed. Chain of custody maintained from collection to lab.
Step 4
Results & Documentation
Lab-confirmed results typically within 24–48 hours. MRO review on all non-negative results. Full documentation delivered for your compliance records.
4

DOT Testing Questions

Frequently Asked Questions

Who is required to have DOT drug testing?

Any employee in a safety-sensitive position regulated by a DOT agency — including FMCSA (CDL drivers), FAA (aviation), FTA (transit), FRA (railroad), PHMSA (pipeline), and USCG (maritime workers) — is required to participate in a DOT drug and alcohol testing program.

Can I use a non-DOT drug test for my CDL drivers?

No. DOT-regulated employees must use federally compliant DOT testing procedures. A non-DOT test does not satisfy federal requirements and will not hold up to a DOT audit.

What happens if a driver tests positive?

A driver who tests positive on a DOT drug test is immediately removed from safety-sensitive duties. They must be evaluated by a Substance Abuse Professional (SAP) and complete a return-to-duty process before resuming DOT-regulated work.

How quickly do you respond for post-accident testing?

We are on-call 24/7. For post-accident situations, we prioritize immediate response. DOT requires alcohol testing within 8 hours and drug testing within 32 hours of a qualifying accident.

What is a DOT random consortium and do I need one?

Yes, if you are a DOT-regulated employer you are required to participate in a random drug and alcohol testing program. A consortium pools multiple employers together for random draws. We can enroll your drivers and manage the entire program.

Ready to Schedule Your

DOT Test?

Call or text Mesha we'll schedule your DOT collection, enroll your drivers in our random consortium, or dispatch a collector for post-accident testing. Same-day and 24/7 available.

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